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Introduction of Principle of SPAD-502 Chlorophyll Content Tester
Introduction of Principle of SPAD-502 Chlorophyll Content Tester
Spad value refers to the relative content of chlorophyll or “greenness degreeâ€. By knowing the spad value, we can understand the true nitro requirement of the plant, and at the same time, we can also judge the amount of nitrogen fertilizer. Chlorophyll is the main substance that absorbs light, and the padding value is related to the chlorophyll content of the plant. That is, if we can determine the chlorophyll content in the plant, we can know the padd value, which is proportional to the nitrogen content in the leaf. . In this way, we can infer the nitrogen content of the plant, and then guide whether the plant needs to apply fertilizer.
Different wavelengths of light, the absorption of the leaves is different, we can determine the relative amount of the current chlorophyll of the leaf through the two wavelength optical density difference mode 650nm and 940nm (transmission coefficient in the two wavelength ranges to determine the current chlorophyll of the leaf Relative quantity). Using this principle, the SPAD-502 Chlorophyll Content Analyzer was born. The SPAD-502Plus measures the relative amount of chlorophyll in the current leaf by measuring the absorption of leaves in two wavelength bands. The figure below shows the absorbance of the spectrum of chlorophyll in the two leaf samples.
It can be seen from the figure that the absorption of chlorophyll in the blue region (400-500 nm) and the red region (600-700 nm) reaches its peak, but it does not absorb in the near-infrared region. In other words, the absorption of chlorophyll is different for different wavelengths of light. This is exactly in line with our requirements. The value of paddled is measured by different absorption.
Using this absorption characteristic of chlorophyll, the SPAD-502 chlorophyll content meter measures the absorption of leaves in the red and near-infrared regions. A SPAD value is calculated from the absorptivity of these two regions, which is a numerical representation of the parameters corresponding to the chlorophyll content in the leaves.