Recently, one customer questioned the technical department of our company. How can I know the quality of the kit I purchased and whether it is a counterfeit product? Nowadays, many upstream manufacturers use counterfeit kits to injure scientific and research workers, and they simply ignore the rigor of scientific research. So how do we determine the quality of a kit product? Let's take a look at the large exposure of the intrinsic quality testing method of ELISA kits shared by Shanghai Hengyuan Biology Company. First, using interference experiments to discriminate whether the ELISA kit detects the target antigen The specific method is as follows: 1. The recombinant antibody antigen specific for the kit and the detection antibody in the kit are configured as an antibody: the mixed solution of the antibody 1:1 is used. 2. Incubate at 37°C for 15 minutes instead of the original kit 3 follow-up operation in accordance with the kit instructions, plus detection of antibodies, enzyme complexes and substrates 4. After the experiment is completed, the standard curve is tested. If the standard curve is good, the target antigen and the kit antibody do not match. The kit detection antibody is for a non-target antigen. The kit is a fake counterfeit kit. 5. If the standard curve is wireless, it means that the kit detection antibody has been neutralized or interfered with by the target antigen, affecting the detection effect of the actual kit antibody, then the kit detects the antibody against the target antigen. First, when two boxes of different ELISA kits are purchased in the same company, cross-experiments can be used to identify The specific method is as follows: 1. Remove the two coupons purchased from Kit A. 2. Make a standard curve for the standard product of a kit plus kit A. 3. Make a standard curve for the other kit plus kit B standard. 4. Follow-up operation according to the kit instructions, plus detection of antibodies, enzyme complexes and substrates. 5. After the experiment is completed, two standard curves are detected. If the two standard curves are the same, the same indicator detected by the two ELISA kits is used instead of A and B, that is, the kit is a fake counterfeit kit. 6. If the two standard curves are inconsistent, it indicates the different indicators of the two kits. Kit A can only detect A, and B cannot detect B, that is, the kit is a normal ELISA kit. The above content for your reference, the specific ELISA kit quality identification method introduced, if not clear or learn more, you can contact our company at any time for details. Shanghai Hengyuan ELISA kit distributor for everyone to supply high-quality TSZ, R & D brand imported ELISA kits, providing full technical guidance. Holographic Tamper Evident Labels With Honeycomb Holographic Tamper Evident Labels With Honeycomb,Medicine Holographic Sticker,2D / 3D Holographic Sticker,Custom 2D / 3D Holographic Sticker SHENZHEN SENING PACKING MATERIAL CO.,LTD , https://www.seningpacking.com
