Differentiation of different plant cultures using artificial climate incubators Artificial climate incubators are widely used in the scientific research of plants and crops. We have found some differences in the cultivation of different plants. Here we have a brief introduction and analysis. Most plant somatic embryos in artificial climate incubators are less sensitive to changes in light, but some plants are also susceptible to light. It has been reported that light illumination in peanut induced somatic embryos significantly inhibits embryogenesis. Strong white light and blue light in carrot cultures inhibit the development of somatic embryos, but under low-intensity blue light, the induction rate of somatic embryos is higher than that in dark conditions. Red light promotes heart-shaped embryo development. WEATHERWAX et al. believe that photoperiod regulates the levels of endogenous ABA in cells through the use of photofrin, and ultimately affects the occurrence of somatic embryos. In addition, photofrin can also affect the anabolism of cells, and it can also affect the synthesis of polyamines. And regulate the expression of specific genes to control the occurrence of somatic embryos. The study of the four major cassava varieties in this study showed that the induction of lateral bud enlargement in dark conditions is more conducive to the formation of somatic embryos, artificial climate incubators are more conducive to the normalization of multiple anabolic metabolism of explant cells. It is consistent with the findings of Deng Xiangyang et al. that the lateral buds are budded and the lateral buds of the buds are more severely differentiated and are not conducive to the formation of somatic embryos. In plant tissue culture, auxin is important for the control of the induction of adventitious buds. In auxin, IBA is mainly used for rooting culture, or in combination with cytokinin at a relatively low level to promote shoot regeneration and proliferation. This study also demonstrated the positive role of IBA in cassava varieties. Different quality concentrations of IBA had certain effects on the adventitious bud inducibility between lines. For example, compared with SC8 and NZ188, the adventitious bud induction rate was 60.0% and 56.9%, respectively, when the IBA was not added. After the 0.3 mg·L-1 IBA concentration, the induction rate of adventitious buds increased significantly. However, when the mass concentration was 0.5 mg·L-1, the adventitious bud induction rate decreased. When the concentration of IBA reached 0.5 mg·L-1, part of the mature cotyledons showed rooting, inhibiting adventitious buds and adversely affecting the formation of adventitious buds. Therefore, for different cassava genotypes, try to add different concentrations of IBA to improve the adventitious buds of some varieties. When the artificial climate incubator was cultured in vitro by a medium-sized plant, addition of AgNO3 to the medium significantly promoted the formation of somatic embryos and the regeneration of whole plants. It is generally believed that after the wounds are explanted, a large amount of self-protected substances will be generated in the wounded parts. (ie, ethylene), which has an inhibitory effect on the differentiation of cells and the formation of organs, and Ag+ is a very good inhibitor of ethylene activity, preventing or reducing ethylene activity by competitively binding ethylene receptor proteins located on the cell membrane. , so as to relieve or relieve the inhibitory effect of ethylene on explant regeneration. The results of this study are consistent with the report that AgNO3 at a suitable concentration can significantly increase the production of adventitious buds and inhibit the formation of callus from mature embryos of cassava, but the addition of AgNO3 to culture explants takes too long and can also cause explants. The effect was caused and the distortion occurred. This may be due to the high concentration of AgNO3, which produced a stress effect on the explants. Therefore, only adding appropriate concentration of AgNO3 in plant explants can effectively promote the induction of adventitious buds and regeneration of explants. Mountain Bike is designed for crosscountry (hills, path, plains and sand gravel road, etc.) ,born in 1977 in the United States on the west coast of San Francisco. At that time, a group of young people having fun on the hillside had an abrupt fantasy: "if I can ride a bicycle dashed down from the mountain, must be very interesting." Then they started to design the crosscountry bicycle. 2 years later , the mountain bike was officially named. Frame Mountain Bike, Hi-Ten Mountain Bike, Colorful Mountain Bicycle Hangzhou Datasports Equipment Co.,Ltd(Oembicycle) , http://www.oembicycle.com